His (C-terminal) Tag Antibody, IgG1, Clone: [M050], Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: His (C-terminal) Tag Antibody, IgG1, Clone: [M050], Unconjugated, Mouse, Monoclonal
• Artikelnummer: BSS-BSM-70513M
• Hersteller Artikelnummer: bsm-70513M
• Alternativnummer: BSS-BSM-70513M-100
• Hersteller: Bioss
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IHC, IP, WB
• Spezies Reaktivität: Bacteria, Mouse, Rat
• Konjugation: Unconjugated
• Alternative Synonym: His Tag, 6x His Tag, HHHHHH

Produktbeschreibung

Epitope tagging (e.g. His-tag) is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). A variety of plasmids contain DNA that encodes an amino- or carboxy-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. These plasmids facilitate expression of His-tagged recombinant proteins that can be isolated or purified by immobilized metal affinity chromatography. Anti-His Tag antibodies can be used to detect recombinant proteins with the His-Tag in many different immunoassays, including western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, flow cytometry, ELISA, and chromatin immunoprecipitation assays.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [M050]
• Isotyp: IgG1
• Puffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
• Quelle: Clone (M050) was generated from a recombinant protein containing a C-terminal His-tag.
• Target-Kategorie: Anti-His (C-terminal) Tag Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB(1:300-5000), FCM(1:20-100), IP(1-2ug), IHC()