His (C-terminal) Tag Antibody, IgG1, Clone: [M050], Unconjugated, Mouse, Monoclonal
Biozol Catalog Number:
BSS-BSM-70513M
Supplier Catalog Number:
bsm-70513M
Alternative Catalog Number:
BSS-BSM-70513M-100
Manufacturer:
Bioss
Host:
Mouse
Category:
Antikörper
Application:
FC, IHC, IP, WB
Species Reactivity:
Bacteria, Mouse, Rat
Conjugation:
Unconjugated
Alternative Names:
His Tag, 6x His Tag, HHHHHH
Epitope tagging (e.g. His-tag) is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). A variety of plasmids contain DNA that encodes an amino- or carboxy-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. These plasmids facilitate expression of His-tagged recombinant proteins that can be isolated or purified by immobilized metal affinity chromatography. Anti-His Tag antibodies can be used to detect recombinant proteins with the His-Tag in many different immunoassays, including western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, flow cytometry, ELISA, and chromatin immunoprecipitation assays.
Clonality:
Monoclonal
Clone Designation:
[M050]
Isotype:
IgG1
Buffer:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Source:
Clone (M050) was generated from a recombinant protein containing a C-terminal His-tag.
Target:
Anti-His (C-terminal) Tag Antibody
Application Dilute:
WB(1:300-5000), FCM(1:20-100), IP(1-2ug), IHC()
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