PVRL1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB1021
Artikelname: PVRL1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB1021
Hersteller Artikelnummer: orb1021
Alternativnummer: BYT-ORB1021-100,BYT-ORB1021-200,BYT-ORB1021-50
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human PVRL1/CD111/Nectin1 (31-130/517aa)
Konjugation: Unconjugated
Alternative Synonym: CD111, CD111 antigen, CLPED1, ectodermal dysplasia 4(Margarita Island type), ED4, Herpes virus entry mediator C, Herpesvirus entry mediator C, Herpesvirus Ig like receptor, Herpesvirus Ig-like receptor, HIgR, HveC, Nectin 1, Nectin-1, Nectin1, OFC7, OROFACIAL CLEFT 7, Poliovirus receptor related protein 1, poliovirus receptor-like 1, Poliovirus receptor-related protein 1, PRR, PRR1, PVRL 1, PVRL1, PVRL1_HUMAN, PVRR, PVRR1, SK-12.
PVRL1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 54 kDa
UniProt: Q15223
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: NECTIN1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (orb1021) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (orb1021) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Human U87MG cell lysates, Lane 2: Human SH-SY5Y cell lysates, Primary: Anti-PVRL1 (orb1021) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 54 kDa.
Sample: Lane 1: Mouse Urinary bladder tissue lysates, Lane 2: Rat Urinary bladder tissue lysates, Primary: Anti-PVRL1 (orb1021) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 57 kDa.