PVRL1 Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: PVRL1 Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB1021
• Hersteller Artikelnummer: orb1021
• Alternativnummer: BYT-ORB1021-50,BYT-ORB1021-100,BYT-ORB1021-200
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human PVRL1/CD111/Nectin1 (31-130/517aa)
• Konjugation: Unconjugated
• Alternative Synonym: CD111, CLPED1, ED4, HIgR, HV1S, HVEC, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK-12, nectin-1, NECT1_HUMAN, NECTIN1, Herpes virus entry mediator C (Herpesvirus entry mediator C | HveC), Herpesvirus Ig-like receptor (HIgR), Nectin cell adhesion molecule 1, Poliovirus receptor-related protein 1, NECT1_MOUSE, NECT1_PIG,

Produktbeschreibung

PVRL1 Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 54 kDa
• UniProt: Q15223
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: NECTIN1

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Bilder

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (orb1021) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (orb1021) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human U87MG cell lysates, Lane 2: Human SH-SY5Y cell lysates, Primary: Anti-PVRL1 (orb1021) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 54 kDa.

Sample: Lane 1: Mouse Urinary bladder tissue lysates, Lane 2: Rat Urinary bladder tissue lysates, Primary: Anti-PVRL1 (orb1021) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 57 kDa.