PVRL1 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: PVRL1 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB1021
• Supplier Catalog Number: orb1021
• Alternative Catalog Number: BYT-ORB1021-100,BYT-ORB1021-200,BYT-ORB1021-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human PVRL1/CD111/Nectin1 (31-130/517aa)
• Conjugation: Unconjugated
• Alternative Names: CD111, CD111 antigen, CLPED1, ectodermal dysplasia 4(Margarita Island type), ED4, Herpes virus entry mediator C, Herpesvirus entry mediator C, Herpesvirus Ig like receptor, Herpesvirus Ig-like receptor, HIgR, HveC, Nectin 1, Nectin-1, Nectin1, OFC7, OROFACIAL CLEFT 7, Poliovirus receptor related protein 1, poliovirus receptor-like 1, Poliovirus receptor-related protein 1, PRR, PRR1, PVRL 1, PVRL1, PVRL1_HUMAN, PVRR, PVRR1, SK-12.

Product Description

PVRL1 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 54 kDa
• UniProt: Q15223
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: NECTIN1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (orb1021) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PVRL1) Polyclonal Antibody, Unconjugated (orb1021) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human U87MG cell lysates, Lane 2: Human SH-SY5Y cell lysates, Primary: Anti-PVRL1 (orb1021) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 54 kDa.

Sample: Lane 1: Mouse Urinary bladder tissue lysates, Lane 2: Rat Urinary bladder tissue lysates, Primary: Anti-PVRL1 (orb1021) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 57 kDa.