Nectin-3, Mouse, mAb 103-A1 Antibody, Unconjugated, Monoclonal
Artikelnummer:
BYT-ORB1109146
Hersteller Artikelnummer:
orb1109146
Alternativnummer:
BYT-ORB1109146-100
Hersteller:
Biorbyt
Kategorie:
Antikörper
Applikation:
FC, Functional Studies, IA, IF, IHC-Fr, IP
Spezies Reaktivität:
Mouse
Immunogen:
Recombinant mouse nectin-3 extracellular domain (50-400 aa) fused to IgG Fc
Konjugation:
Unconjugated
Alternative Synonym:
PPR3, poliovirus receptor-related protein 3, PRR3, PVRR3, CD113
Mouse monoclonal antibody to Nectin-3
Klonalität:
Monoclonal
Puffer:
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide
Formulierung:
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide
Application Verdünnung:
For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Anwendungsbeschreibung:
Application Notes: IF: fixed in 2% paraformaldehyde in PBS optionally followed by methanol/aceton fixation (Ref 2-7) FC: 1 ug per 1 x 106 cells FS: injection of 1ug into lumen of seminiferous tubules (Ref 7) IA: Indirect ELISA on 0.5µg/ml mouse nectin-3 coated per well. Positive signal with 2µg/ml antibody. IF: Cells are fixed in 2 % paraformaldehyde in PBS, optionally followed by methanol/aceton fixation (Ref 1-6) IP: 1-2 ug per 100-500 ug of total protein (1 ml of cell lysate)
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