Nectin-3, Mouse, mAb 103-A1 Antibody, Unconjugated, Monoclonal
Biozol Catalog Number:
BYT-ORB1109146
Supplier Catalog Number:
orb1109146
Alternative Catalog Number:
BYT-ORB1109146-100
Manufacturer:
Biorbyt
Category:
Antikörper
Application:
FC, Functional Studies, IA, IF, IHC-Fr, IP
Species Reactivity:
Mouse
Immunogen:
Recombinant mouse nectin-3 extracellular domain (50-400 aa) fused to IgG Fc
Conjugation:
Unconjugated
Alternative Names:
PPR3, poliovirus receptor-related protein 3, PRR3, PVRR3, CD113
Mouse monoclonal antibody to Nectin-3
Clonality:
Monoclonal
Buffer:
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide
Form:
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide
Application Dilute:
For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Application Notes:
Application Notes: IF: fixed in 2% paraformaldehyde in PBS optionally followed by methanol/aceton fixation (Ref 2-7) FC: 1 ug per 1 x 106 cells FS: injection of 1ug into lumen of seminiferous tubules (Ref 7) IA: Indirect ELISA on 0.5µg/ml mouse nectin-3 coated per well. Positive signal with 2µg/ml antibody. IF: Cells are fixed in 2 % paraformaldehyde in PBS, optionally followed by methanol/aceton fixation (Ref 1-6) IP: 1-2 ug per 100-500 ug of total protein (1 ml of cell lysate)
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