Featured Anti-GAPDH Mouse Monoclonal Antibody (2B5), specially designed for your immunoassay as internal control.
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Puffer:
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Formulierung:
Liquid solution
Anwendungsbeschreibung:
Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IHC-P (1:400), IF (1:400)
Western blot analysis of Hela (1), rat brain (2), rabbit muscle(3), sheep muscle(4), and mouse brain (5), diluted at 1:10000.
Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4C, overnight). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4C, overnight). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4C, overnight). Negative control was used by secondary antibody only.
Immunofluorescence analysis of human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Immunofluorescence analysis of mouse liver tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Western blot analysis of Pig muscle tissue, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25C, 3h).
Western blot analysis of Hela, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25C, 3h).
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