Anti-GAPDH Mouse Monoclonal Antibody (2B5), Unconjugated

Product Overview

• Article Name: Anti-GAPDH Mouse Monoclonal Antibody (2B5), Unconjugated
• Biozol Catalog Number: BYT-ORB1147864
• Supplier Catalog Number: orb1147864
• Alternative Catalog Number: BYT-ORB1147864-200,BYT-ORB1147864-5,BYT-ORB1147864-50
• Manufacturer: Biorbyt
• Category: Antikörper
• Application: IF, IHC-P, WB
• Species Reactivity: Canine, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Yeast
• Immunogen: Synthetic Peptide
• Conjugation: Unconjugated
• Alternative Names: GAPDH, GAPD, CDABP0047, OK/SW-cl.12, Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH

Product Description

Featured Anti-GAPDH Mouse Monoclonal Antibody (2B5), specially designed for your immunoassay as internal control.

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Buffer: Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
• Form: Liquid solution

Application Notes

• Application Notes: Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IHC-P (1:400), IF (1:400)

Images

Western blot analysis of Hela (1), rat brain (2), rabbit muscle(3), sheep muscle(4), and mouse brain (5), diluted at 1:10000.

Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4C, overnight). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4C, overnight). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4C, overnight). Negative control was used by secondary antibody only.

Immunofluorescence analysis of human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Immunofluorescence analysis of mouse liver tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Western blot analysis of Pig muscle tissue, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25C, 3h).

Western blot analysis of Hela, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25C, 3h).