Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Target-Kategorie:
ZAP70
Anwendungsbeschreibung:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kDa according to NP_001070.2). Recommended concentration: 0.3-1µg/mL.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer). Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371
orb1248728 (0.3 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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