ZAP70 Antibody, Unconjugated, Goat, Polyclonal

Product Overview

• Article Name: ZAP70 Antibody, Unconjugated, Goat, Polyclonal
• Biozol Catalog Number: BYT-ORB1248728
• Supplier Catalog Number: orb1248728
• Alternative Catalog Number: BYT-ORB1248728-0.1
• Manufacturer: Biorbyt
• Host: Goat
• Category: Antikörper
• Application: ELISA, IP, WB
• Species Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SDGYTPEPARITSPD
• Conjugation: Unconjugated
• Alternative Names: ZAP70, zeta-chain (TCR) associated protein kinase 70kDa, FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70, 70 kDa zeta-associated protein, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, zeta-chain associated protein kinase, 70kD

Product Description

ZAP70 Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 500 ug/mL
• Molecular Weight: Approx 70 kDa
• NCBI: 001070
• UniProt: P43403
• Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
• Form: Liquid
• Target: ZAP70

Application Notes

• Application Notes: Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kDa according to NP_001070.2). Recommended concentration: 0.3-1µg/mL.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer). Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371

Images

orb1248728 (0.3 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.