GFAP Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1259841
Artikelname: GFAP Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1259841
Hersteller Artikelnummer: orb1259841
Alternativnummer: BYT-ORB1259841-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1).
Konjugation: Unconjugated
Alternative Synonym: Glial fibrillary acidic protein, GFAP, GFAP
GFAP Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 50kDa
UniProt: P14136
Puffer: Liquid
Formulierung: Liquid
Target-Kategorie: GFAP
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using GFAP antibody (orb1259841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using GFAP antibody (orb1259841) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP antibody (orb1259841) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GFAP antibody (orb1259841) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.