Western blot analysis of extracts of various cell lines, using GFAP antibody (orb1259841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using GFAP antibody (orb1259841) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP antibody (orb1259841) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GFAP antibody (orb1259841) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.
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