GFAP Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: GFAP Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB1259841
• Supplier Catalog Number: orb1259841
• Alternative Catalog Number: BYT-ORB1259841-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1).
• Conjugation: Unconjugated
• Alternative Names: Glial fibrillary acidic protein, GFAP, GFAP

Product Description

GFAP Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 50kDa
• UniProt: P14136
• Buffer: Liquid
• Form: Liquid
• Target: GFAP

Application Notes

• Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using GFAP antibody (orb1259841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat brain using GFAP antibody (orb1259841) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP antibody (orb1259841) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using GFAP antibody (orb1259841) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U-251MG cells using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat brain using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse brain using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.