TEAD1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1260451
Artikelname: TEAD1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1260451
Hersteller Artikelnummer: orb1260451
Alternativnummer: BYT-ORB1260451-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ChIP, IF, IHC, WB
Spezies Reaktivität: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).
Konjugation: Unconjugated
Alternative Synonym: TEAD1, AA, REF1, TCF13, TEF-1, NTEF-1, TCF-13, TEAD-1
TEAD1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 48kDa
UniProt: P28347
Puffer: Liquid
Formulierung: Liquid
Target-Kategorie: TEAD1
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200CHIP: 1:20 - 1:50
Western blot analysis of extracts of various cell lines, using TEAD1 antibody (orb1260451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
Immunohistochemistry of paraffin-embedded human lung cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (orb1260451).
Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody (orb1260451) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.