TEAD1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1260451
Article Name: TEAD1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1260451
Supplier Catalog Number: orb1260451
Alternative Catalog Number: BYT-ORB1260451-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: ChIP, IF, IHC, WB
Species Reactivity: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).
Conjugation: Unconjugated
Alternative Names: TEAD1, AA, REF1, TCF13, TEF-1, NTEF-1, TCF-13, TEAD-1
TEAD1 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 48kDa
UniProt: P28347
Buffer: Liquid
Form: Liquid
Target: TEAD1
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200CHIP: 1:20 - 1:50
Western blot analysis of extracts of various cell lines, using TEAD1 antibody (orb1260451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
Immunohistochemistry of paraffin-embedded human lung cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using TEAD1 antibody (orb1260451) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (orb1260451).
Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody (orb1260451) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.