Western blot analysis of extracts of various cell lines, using BRCA1 antibody (orb1261228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of C6 cells using c-Jun antibody (orb1261262) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse pancreas using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
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