BRCA1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1261228
Article Name: BRCA1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1261228
Supplier Catalog Number: orb1261228
Alternative Catalog Number: BYT-ORB1261228-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide of human BRCA1
Conjugation: Unconjugated
Alternative Names: Breast cancer type 1 susceptibility protein, 632-, RING finger protein 53, BRCA1, RNF53
BRCA1 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 300kDa
UniProt: P38398
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: BRCA1
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using BRCA1 antibody (orb1261228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of C6 cells using c-Jun antibody (orb1261262) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse pancreas using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).