Tnf Antibody (Biotin), Rabbit, Polyclonal

Artikelnummer: BYT-ORB1272286
Artikelname: Tnf Antibody (Biotin), Rabbit, Polyclonal
Artikelnummer: BYT-ORB1272286
Hersteller Artikelnummer: orb1272286
Alternativnummer: BYT-ORB1272286-0.05
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Mouse
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mTNF-alpha (murine TNF-alpha).
Konjugation: Biotin
Alternative Synonym: DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tnfsf2, Tumor necrosis factor, Cachectin
Tnf Antibody (Biotin)
Klonalität: Polyclonal
Konzentration: batch dependent
NCBI: 06804
UniProt: P06804
Formulierung: Lyophilized
Target-Kategorie: Tnf
Anwendungsbeschreibung: Application Notes: ELISA:Sandwich:To detect mTNF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine TNF-alpha (XP-5284) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mTNF-alpha.Western Blot:To detect mTNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mTNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
To detect Murine TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 - 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Murine TNF-alpha (orb1272287) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF-alpha.
To detect Murine TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine TNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Murine TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine TNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.