Application Notes: ELISA:Sandwich:To detect mTNF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine TNF-alpha (XP-5284) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mTNF-alpha.Western Blot:To detect mTNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mTNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
To detect Murine TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 - 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Murine TNF-alpha (orb1272287) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF-alpha.
To detect Murine TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine TNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Murine TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine TNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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