LMNB1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: LMNB1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: BYT-ORB1274472
• Hersteller Artikelnummer: orb1274472
• Alternativnummer: BYT-ORB1274472-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 397-586 of human Lamin B1 (NP_005564.1).
• Konjugation: Unconjugated
• Alternative Synonym: LMNB1, lamin B1, ADLD, LMN, LMN2, LMNB, MGC111419, OTTHUMP00000159218, lamin-B1

Produktbeschreibung

LMNB1 Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 70kDa
• UniProt: P20700
• Puffer: Liquid
• Formulierung: Liquid
• Target-Kategorie: LMNB1

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (orb1274472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from normal (control) and Lamin B1 knockout (KO) HeLa cells, using Lamin B1 antibody (orb1274472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat spinal cord using Lamin B1 antibody (orb1274472) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using Lamin B1 antibody (orb1274472) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using Lamin B1 Polyclonal Antibody (orb1274472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Lamin B1 Polyclonal Antibody (orb1274472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.