LMNB1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: LMNB1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB1274472
• Supplier Catalog Number: orb1274472
• Alternative Catalog Number: BYT-ORB1274472-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 397-586 of human Lamin B1 (NP_005564.1).
• Conjugation: Unconjugated
• Alternative Names: LMNB1, lamin B1, ADLD, LMN, LMN2, LMNB, MGC111419, OTTHUMP00000159218, lamin-B1

Product Description

LMNB1 Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 70kDa
• UniProt: P20700
• Buffer: Liquid
• Form: Liquid
• Target: LMNB1

Application Notes

• Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (orb1274472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from normal (control) and Lamin B1 knockout (KO) HeLa cells, using Lamin B1 antibody (orb1274472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat spinal cord using Lamin B1 antibody (orb1274472) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using Lamin B1 antibody (orb1274472) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using Lamin B1 Polyclonal Antibody (orb1274472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Lamin B1 Polyclonal Antibody (orb1274472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.