CD3 Intracellular domain Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: CD3 Intracellular domain Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: BYT-ORB1294242
• Hersteller Artikelnummer: orb1294242
• Alternativnummer: BYT-ORB1294242-100,BYT-ORB1294242-25
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human/mouse CD3
• Konjugation: Unconjugated

Produktbeschreibung

Rabbit polyclonal antibody to CD3 (Intracellular

Produkteigenschaften

• Klonalität: Polyclonal
• Target-Kategorie: CD3 Intracellular domain

Anwendungsbeschreibung

• Application Verdünnung: Western Blot 1:500-1:1000 Immunofluorescence 1:300-1:400 Immunohistochemistry (Paraffin) 1:100-1:300

Bilder

Anti-CD3 antibody at 1/1000 dilution. Lysates at 40 µg per lane. This blot was produced using a 15% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with orb1294242 overnight at 4C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294242 anti-CD3 antibody at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human Hepatocellular cancer tissue labeling CD3 with orb1294242 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Tonsil carcinoma tissue labeling CD3 with orb1294242 at 1/200.

Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with orb1294242 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with orb1294242 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse spleen labeling CD3 with orb1294242 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

CD3 antibody, orb1294242, Sample: Human peripheral blood, anti-CD3 antibody at 1:100.