LC3B Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: LC3B Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB1294361
• Hersteller Artikelnummer: orb1294361
• Alternativnummer: BYT-ORB1294361-25,BYT-ORB1294361-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Synthetic peptide / encompassing a sequence within the N-terminus region.
• Konjugation: Unconjugated

Produktbeschreibung

Rabbit polyclonal antibody to LC3B

Produkteigenschaften

• Klonalität: Polyclonal
• Puffer: 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Target-Kategorie: LC3B

Anwendungsbeschreibung

• Application Verdünnung: Western Blot 1:1000 - 1:2000, Immunofluorescence 1:200 - 1:500, Immunohistochemistry (Paraffin) 1:100 - 1:200

Bilder

All lanes: Anti-LC3B antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294361 overnight at 4C.

LC3B antibody at 1/1000 dilution. Lysates/proteins at 50 µg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294361 overnight at 4C.

LC3B antibody at 1/1000 dilution. Lysates/proteins at 50 µg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294361 overnight at 4C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294361 anti-LC3B antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294361 anti-LC3B antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human colorental and liver cancer tissue labeling LC3B with orb1294361 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Kidney cancer tissue labeling LC3B with orb1294361 at 1/100.

Immunohistochemical analysis of paraffin embedded Human testis and breast cancer tissue labeling LC3B with orb1294361 at 1/100.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling LC3B with orb1294361 at 1/200.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling LC3B with orb1294361 at 1/200.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling LC3B with orb1294361 at 1/200.