LC3B Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB1294361
- Images (11)
| Article Name: | LC3B Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB1294361 |
| Supplier Catalog Number: | orb1294361 |
| Alternative Catalog Number: | BYT-ORB1294361-25,BYT-ORB1294361-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Synthetic peptide / encompassing a sequence within the N-terminus region. |
| Conjugation: | Unconjugated |
| Rabbit polyclonal antibody to LC3B |
| Clonality: | Polyclonal |
| Buffer: | 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Target: | LC3B |
| Application Dilute: | Western Blot 1:1000 - 1:2000, Immunofluorescence 1:200 - 1:500, Immunohistochemistry (Paraffin) 1:100 - 1:200 |
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All lanes: Anti-LC3B antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294361 overnight at 4C. |
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LC3B antibody at 1/1000 dilution. Lysates/proteins at 50 µg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294361 overnight at 4C. |
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LC3B antibody at 1/1000 dilution. Lysates/proteins at 50 µg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294361 overnight at 4C. |
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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294361 anti-LC3B antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain. |
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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294361 anti-LC3B antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain. |
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Immunohistochemical analysis of paraffin embedded Human colorental and liver cancer tissue labeling LC3B with orb1294361 at 1/100. |
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Immunohistochemical analysis of paraffin embedded Human Kidney cancer tissue labeling LC3B with orb1294361 at 1/100. |
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Immunohistochemical analysis of paraffin embedded Human testis and breast cancer tissue labeling LC3B with orb1294361 at 1/100. |
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Immunohistochemical analysis of paraffin embedded mouse tissue labeling LC3B with orb1294361 at 1/200. |
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Immunohistochemical analysis of paraffin embedded mouse tissue labeling LC3B with orb1294361 at 1/200. |
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Immunohistochemical analysis of paraffin embedded mouse tissue labeling LC3B with orb1294361 at 1/200. |
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