RNA polymerase II Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: RNA polymerase II Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB1312
• Hersteller Artikelnummer: orb1312
• Alternativnummer: BYT-ORB1312-100,BYT-ORB1312-200,BYT-ORB1312-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P
• Spezies Reaktivität: Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Pol II/RNA polymerase II (101-200/1970aa)
• Konjugation: Unconjugated
• Alternative Synonym: POLR2A, POLR2, DNA directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit, DNA-directed RNA polymerase II subunit A, RPB1_HUMAN.

Produktbeschreibung

RNA polymerase II Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 217 kDa
• UniProt: P24928
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: POLR2A

Anwendungsbeschreibung

• Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Bilder

Paraformaldehyde-fixed, paraffin embedded (mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (orb1312) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (orb1312) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (orb1312) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (orb1312) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (orb1312) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (orb1312) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-POLR2 Polyclonal Antibody, Unconjugated (orb1312) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Immunohistochemical staining of mouse intestine tissue using Pol II antibody.