RNA polymerase II Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: RNA polymerase II Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB1312
• Supplier Catalog Number: orb1312
• Alternative Catalog Number: BYT-ORB1312-100,BYT-ORB1312-200,BYT-ORB1312-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P
• Species Reactivity: Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Pol II/RNA polymerase II (101-200/1970aa)
• Conjugation: Unconjugated
• Alternative Names: POLR2A, POLR2, DNA directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit, DNA-directed RNA polymerase II subunit A, RPB1_HUMAN.

Product Description

RNA polymerase II Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 217 kDa
• UniProt: P24928
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: POLR2A

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (orb1312) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (orb1312) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (orb1312) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (orb1312) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (orb1312) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (orb1312) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-POLR2 Polyclonal Antibody, Unconjugated (orb1312) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Immunohistochemical staining of mouse intestine tissue using Pol II antibody.