Human Anti-Hepatitis C Antibody (Anti-HCV) ELISA Kit
Artikelnummer:
BYT-ORB1496520
Hersteller Artikelnummer:
orb1496520
Alternativnummer:
BYT-ORB1496520-48,BYT-ORB1496520-96
Hersteller:
Biorbyt
Kategorie:
Kits/Assays
Spezies Reaktivität:
Human
Alternative Synonym:
Hepatitis C virus IgG
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human anti-HCV. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human anti-HCV. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human anti-HCV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human anti-HCV in the samples is then determined by comparing the OD of the samples to the standard curve.
Konzentration:
20 ng/mL
Detektionsbereich:
0.32-20 ng/mL
Sensitivitaet:
0.12 ng/mL
Proben:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung:
Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human anti-HCV. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human anti-HCV. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human anti-HCV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human anti-HCV in the samples is then determined by comparing the OD of the samples to the standard curve
orb1496520
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