Human Anti-Hepatitis C Antibody (Anti-HCV) ELISA Kit
Biozol Catalog Number:
BYT-ORB1496520
Supplier Catalog Number:
orb1496520
Alternative Catalog Number:
BYT-ORB1496520-48,BYT-ORB1496520-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
Hepatitis C virus IgG
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human anti-HCV. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human anti-HCV. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human anti-HCV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human anti-HCV in the samples is then determined by comparing the OD of the samples to the standard curve.
Concentration:
20 ng/mL
Range:
0.32-20 ng/mL
Sensitivity:
0.12 ng/mL
Samples:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human anti-HCV. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human anti-HCV. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human anti-HCV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human anti-HCV in the samples is then determined by comparing the OD of the samples to the standard curve
orb1496520
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