PTOV1 Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: PTOV1 Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB158227
• Hersteller Artikelnummer: orb158227
• Alternativnummer: BYT-ORB158227-100,BYT-ORB158227-200,BYT-ORB158227-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human PTOV1 (61-160/416aa)
• Konjugation: Unconjugated
• Alternative Synonym: ACID2, Activator interaction domain containing protein 2, Gcap3, prostate tumor overexpressed 1, Prostate tumor overexpressed gene 1, PTOV 1, PTOV1_HUMAN, Prostate tumor-overexpressed gene 1 protein, PTOV-1, Activator interaction domain-containing protein 2.

Produktbeschreibung

PTOV1 Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 47 kDa
• UniProt: Q86YD1
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: PTOV1

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Bilder

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTOV1) Polyclonal Antibody, Unconjugated (orb158227) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTOV1) Polyclonal Antibody, Unconjugated (orb158227) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTOV1) Polyclonal Antibody, Unconjugated (orb158227) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTOV1) Polyclonal Antibody, Unconjugated (orb158227) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTOV1) Polyclonal Antibody, Unconjugated (orb158227) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Lane 3: Rat Stomach tissue lysates, Primary: Anti-PTOV1 (orb158227) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 45 kDa.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-PTOV1 (orb158227) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 48 kD.

IHC-P analysis of rat stomach using PTOV1 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of human rectal carcinoma using PTOV1 Polyclonal Antibody at 1:200 dilution.