PTOV1 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: PTOV1 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB158227
• Supplier Catalog Number: orb158227
• Alternative Catalog Number: BYT-ORB158227-100,BYT-ORB158227-200,BYT-ORB158227-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human PTOV1 (61-160/416aa)
• Conjugation: Unconjugated
• Alternative Names: ACID2, Activator interaction domain containing protein 2, Gcap3, prostate tumor overexpressed 1, Prostate tumor overexpressed gene 1, PTOV 1, PTOV1_HUMAN, Prostate tumor-overexpressed gene 1 protein, PTOV-1, Activator interaction domain-containing protein 2.

Product Description

PTOV1 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 47 kDa
• UniProt: Q86YD1
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: PTOV1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTOV1) Polyclonal Antibody, Unconjugated (orb158227) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTOV1) Polyclonal Antibody, Unconjugated (orb158227) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTOV1) Polyclonal Antibody, Unconjugated (orb158227) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTOV1) Polyclonal Antibody, Unconjugated (orb158227) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTOV1) Polyclonal Antibody, Unconjugated (orb158227) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Lane 3: Rat Stomach tissue lysates, Primary: Anti-PTOV1 (orb158227) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 45 kDa.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-PTOV1 (orb158227) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 48 kD.

IHC-P analysis of rat stomach using PTOV1 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of human rectal carcinoma using PTOV1 Polyclonal Antibody at 1:200 dilution.