TAME is a cell-permeable inhibitor of the Anaphase-Promoting Complex/Cyclosome (APC/C). It functions by competitively blocking the binding of the activators Cdc20 and Cdh1, thereby inhibiting APC/C-dependent proteolysis. This compound is a valuable tool for studying cell cycle regulation, mitotic exit, and aneuploidy in both cellular and biochemical assays.