General 5-Hydroxyindoleacetic Acid (5-HIAA) ELISA Kit

Produktübersicht

• Artikelname: General 5-Hydroxyindoleacetic Acid (5-HIAA) ELISA Kit
• Artikelnummer: BYT-ORB1806512
• Hersteller Artikelnummer: orb1806512
• Alternativnummer: BYT-ORB1806512-48,BYT-ORB1806512-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Spezies Reaktivität: All
• Alternative Synonym: 5HIAA

Produktbeschreibung

General 5-Hydroxyindoleacetic Acid (5-HIAA) ELISA Kit

Produkteigenschaften

• Detektionsbereich: 6.25-400ng/mL
• Sensitivitaet: 1.93 ng/mL
• Proben: serum, plasma, urine, cerebrospinal fluid and other biological fluids
• Target-Kategorie: 5-HIAA

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxyindoleacetic Acid(5-HIAA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxyindoleacetic Acid(5-HIAA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of 5-Hydroxyindoleacetic Acid(5-HIAA) in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

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