General 5-Hydroxyindoleacetic Acid (5-HIAA) ELISA Kit
Biozol Catalog Number:
BYT-ORB1806512
Supplier Catalog Number:
orb1806512
Alternative Catalog Number:
BYT-ORB1806512-48,BYT-ORB1806512-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Species Reactivity:
All
Alternative Names:
5HIAA
General 5-Hydroxyindoleacetic Acid (5-HIAA) ELISA Kit
Range:
6.25-400ng/mL
Sensitivity:
1.93 ng/mL
Samples:
serum, plasma, urine, cerebrospinal fluid and other biological fluids
Target:
5-HIAA
Application Notes:
Application Notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxyindoleacetic Acid(5-HIAA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxyindoleacetic Acid(5-HIAA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of 5-Hydroxyindoleacetic Acid(5-HIAA) in the samples is then determined by comparing the OD of the samples to the standard curve
orb1806512
orb1806512
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