HSP27 Antibody (Biotin), Clone: [8A7], Mouse, Monoclonal

Produktübersicht

• Artikelname: HSP27 Antibody (Biotin), Clone: [8A7], Mouse, Monoclonal
• Artikelnummer: BYT-ORB181808
• Hersteller Artikelnummer: orb181808
• Alternativnummer: BYT-ORB181808-100
• Hersteller: Biorbyt
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FACS, FC, ICC, IF, IHC, IP, WB
• Spezies Reaktivität: Bovine, Canine, Guinea pig, Hamster, Human, Mouse, Rat, Sheep
• Immunogen: HSP27 peptide
• Konjugation: Biotin
• Alternative Synonym: HSPB1, HSP27, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, MKBP, DMPK-binding protein

Produktbeschreibung

Mouse monoclonal antibody against Hsp27, conjugated to Biotin.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml
• Klon-Bezeichnung: [8A7]
• Molekulargewicht: 27kDa
• NCBI: 001532
• UniProt: P04792
• Puffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target-Kategorie: HSP27

Anwendungsbeschreibung

• Application Verdünnung: WB (1:5000), ICC/IF (1:200), IHC (1:100)
• Anwendungsbeschreibung: Application Notes: A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis

Bilder

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Investigation at 11 days gestation.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Western Blot analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Load: 15 µg. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.