HSP27 Antibody (Biotin), Clone: [8A7], Mouse, Monoclonal

Product Overview

• Article Name: HSP27 Antibody (Biotin), Clone: [8A7], Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB181808
• Supplier Catalog Number: orb181808
• Alternative Catalog Number: BYT-ORB181808-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: FACS, FC, ICC, IF, IHC, IP, WB
• Species Reactivity: Bovine, Canine, Guinea pig, Hamster, Human, Mouse, Rat, Sheep
• Immunogen: HSP27 peptide
• Conjugation: Biotin
• Alternative Names: HSPB1, HSP27, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, MKBP, DMPK-binding protein

Product Description

Mouse monoclonal antibody against Hsp27, conjugated to Biotin.

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [8A7]
• Molecular Weight: 27kDa
• NCBI: 001532
• UniProt: P04792
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target: HSP27

Application Notes

• Application Dilute: WB (1:5000), ICC/IF (1:200), IHC (1:100)
• Application Notes: Application Notes: A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis

Images

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Investigation at 11 days gestation.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Western Blot analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Load: 15 µg. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.