Actin Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: Actin Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB182438
• Hersteller Artikelnummer: orb182438
• Alternativnummer: BYT-ORB182438-50,BYT-ORB182438-100,BYT-ORB182438-200
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human ACTA1 (3-100/377aa)
• Konjugation: Unconjugated
• Alternative Synonym: ACTA, ASMA, CFTD, CFTD1, CFTDM, CMYO2A, CMYO2B, CMYO2C, CMYP2A, CMYP2B, CMYP2C, MPFD, NEM1, NEM2, NEM3, SHPM, Acta-2, Acts, Actsk-1, ACTS_CHICK, ACTA1, Alpha-actin-1, 3.6.4.-, ACTS_BOVIN, ACTS_HUMAN, ACTS_MOUSE, ACTS_PIG, ACTS_RABIT, ACTS_RAT,

Produktbeschreibung

Actin Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 42 kDa
• Pubmed: 29709653
• UniProt: P68133
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: ACTA1

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Bilder

Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (orb182438) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (orb182438) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (orb182438) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Bone (Mouse) Lysate at 40 ug, Primary: Anti-Actin (orb182438) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Actin (orb182438) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Actin (orb182438) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

IHC-P analysis of mouse skeletal muscle using Actin Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of rat skeletal muscle using Actin Polyclonal Antibody at 1:200 dilution.