Actin Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Actin Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB182438
• Supplier Catalog Number: orb182438
• Alternative Catalog Number: BYT-ORB182438-100,BYT-ORB182438-200,BYT-ORB182438-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human ACTA1 (3-100/377aa)
• Conjugation: Unconjugated
• Alternative Names: muscle Actin, ACTS_HUMAN, Actin, alpha skeletal muscle, alpha-actin-1, ACTA1, ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, Actin alpha skeletal muscle, actin, alpha 1, skeletal muscle 1, actin, alpha 1, skeletal muscle, actina, aktin, alpha Actin 1, alpha skeletal muscle Actin, alpha skeletal muscle, alpha-actin, beta cytoskeletal actin, nemaline myopathy type 3.

Product Description

Actin Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 42 kDa
• Pubmed: 29709653
• UniProt: P68133
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: ACTA1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (orb182438) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (orb182438) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (orb182438) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Bone (Mouse) Lysate at 40 ug, Primary: Anti-Actin (orb182438) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Actin (orb182438) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Actin (orb182438) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

IHC-P analysis of mouse skeletal muscle using Actin Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of rat skeletal muscle using Actin Polyclonal Antibody at 1:200 dilution.