LNX2 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB184982
Artikelname: LNX2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB184982
Hersteller Artikelnummer: orb184982
Alternativnummer: BYT-ORB184982-100,BYT-ORB184982-200,BYT-ORB184982-50
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: KLH conjugated synthetic peptide derived from human LNX2 (401-500/690aa)
Konjugation: Unconjugated
Alternative Synonym: FLJ12933, FLJ23932, FLJ38000, Ligand of Numb protein X 2, LNX 2, LNX2, LNX2_HUMAN, MGC46315, Numb binding protein 2, Numb-binding protein 2, PDZ domain containing ring finger 1, PDZ domain containing RING finger protein 1, PDZ domain-containing RING finger protein 1, PDZRN 1, PDZRN1.
LNX2 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 76 kDa
UniProt: Q8N448
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: LNX2
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LNX2) Polyclonal Antibody, Unconjugated (orb184982) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-LNX2 (orb184982) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 76 kD, Observed band size: 76 kD.