KLH conjugated synthetic peptide derived from human LNX2 (401-500/690aa)
Conjugation:
Unconjugated
Alternative Names:
FLJ12933, FLJ23932, FLJ38000, Ligand of Numb protein X 2, LNX 2, LNX2, LNX2_HUMAN, MGC46315, Numb binding protein 2, Numb-binding protein 2, PDZ domain containing ring finger 1, PDZ domain containing RING finger protein 1, PDZ domain-containing RING finger protein 1, PDZRN 1, PDZRN1.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LNX2) Polyclonal Antibody, Unconjugated (orb184982) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-LNX2 (orb184982) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 76 kD, Observed band size: 76 kD.
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