LW1/HSFX1 Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: LW1/HSFX1 Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB185091
• Hersteller Artikelnummer: orb185091
• Alternativnummer: BYT-ORB185091-100,BYT-ORB185091-200,BYT-ORB185091-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human
• Immunogen: KLH conjugated synthetic peptide derived from human LW1/HSFX1 (181-280/423aa)
• Konjugation: Unconjugated
• Alternative Synonym: hCG_27154, heat shock transcription factor family X linked 1, Heat shock transcription factor, X-linked, HSFX1, HSFX2, LW 1, LW1.

Produktbeschreibung

LW1/HSFX1 Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 45 kDa
• UniProt: Q9UBD0
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: HSFX1

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Bilder

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSFX1) Polyclonal Antibody, Unconjugated (orb185091) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Jurkat Cell (Human) Lysate at 40 ug, Hela Cell (Human) Lysate at 40 ug, Primary: Anti-LW1/HSFX1 (orb185091) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 48 kD.