LW1/HSFX1 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: LW1/HSFX1 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB185091
• Supplier Catalog Number: orb185091
• Alternative Catalog Number: BYT-ORB185091-100,BYT-ORB185091-200,BYT-ORB185091-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human
• Immunogen: KLH conjugated synthetic peptide derived from human LW1/HSFX1 (181-280/423aa)
• Conjugation: Unconjugated
• Alternative Names: hCG_27154, heat shock transcription factor family X linked 1, Heat shock transcription factor, X-linked, HSFX1, HSFX2, LW 1, LW1.

Product Description

LW1/HSFX1 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 45 kDa
• UniProt: Q9UBD0
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: HSFX1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSFX1) Polyclonal Antibody, Unconjugated (orb185091) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Jurkat Cell (Human) Lysate at 40 ug, Hela Cell (Human) Lysate at 40 ug, Primary: Anti-LW1/HSFX1 (orb185091) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 48 kD.