Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ZC3HAV1L) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Lung tissue lysates, Lane 3: Mouse NIH/3T3 cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human HepG2 cell lysates, Lane 6: Human HeLa cell lysates, Primary: Anti-LYPLA3 (orb185108) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kDa, Observed band size: 48 kDa.
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