LYPLA3 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: LYPLA3 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB185108
• Supplier Catalog Number: orb185108
• Alternative Catalog Number: BYT-ORB185108-100,BYT-ORB185108-200,BYT-ORB185108-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse
• Immunogen: KLH conjugated synthetic peptide derived from human LYPLA3 (101-200/412aa)
• Conjugation: Unconjugated
• Alternative Names: 1 O acylceramide synthase, 1-O-acylceramide synthase, ACS, DKFZp564A0122, Group XV phospholipase A2, GXVPLA 2, GXVPLA2, LCAT like lysophospholipase, LCAT-like lysophospholipase, LLPL, LPLA 2, LPLA2, LYPLA 3, Lysophospholipase 3(lysosomal phospholipase A2), Lysophospholipase 3, Lysosomal phospholipase A2, OTTHUMP00000174859, PAG15_HUMAN, phospholipase A2, group XV, PLA2G15, UNQ341/PRO540.

Product Description

LYPLA3 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 43 kDa
• UniProt: Q8NCC3
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: PLA2G15

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ZC3HAV1L) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Lung tissue lysates, Lane 3: Mouse NIH/3T3 cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human HepG2 cell lysates, Lane 6: Human HeLa cell lysates, Primary: Anti-LYPLA3 (orb185108) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kDa, Observed band size: 48 kDa.