Phospho-MNK1 (Thr385) Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB185175
Artikelname: Phospho-MNK1 (Thr385) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB185175
Hersteller Artikelnummer: orb185175
Alternativnummer: BYT-ORB185175-100,BYT-ORB185175-200,BYT-ORB185175-50
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P
Spezies Reaktivität: Human
Immunogen: KLH conjugated synthesised phosphopeptide derived from human MNK1 around the phosphorylation site of Thr385 LP(p-T)P
Konjugation: Unconjugated
Alternative Synonym: MNK1(phospho T385), p-MNK1(phospho T385), MAP kinase interacting kinase 1, MAP kinase interacting serine/threonine kinase 1, MAP kinase signal integrating kinase 1, MAP kinase signal-integrating kinase 1, MAP kinase-interacting serine/threonine-protein kinase 1, MAPK signal integrating kinase 1, MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1, MKNK1, MKNK1_HUMAN, MNK 1, Mnk1.
Phospho-MNK1 (Thr385) Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 51 kDa
UniProt: Q9BUB5
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: MKNK1
Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Anwendungsbeschreibung: Modification: Phosphorylated
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-MNK1 (Thr385)) Polyclonal Antibody, Unconjugated (orb185175) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p-MNK1 (Thr385)) Polyclonal Antibody, Unconjugated (orb185175) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.