Phospho-MNK1 (Thr385) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-MNK1 (Thr385) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB185175
• Supplier Catalog Number: orb185175
• Alternative Catalog Number: BYT-ORB185175-100,BYT-ORB185175-200,BYT-ORB185175-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P
• Species Reactivity: Human
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human MNK1 around the phosphorylation site of Thr385 LP(p-T)P
• Conjugation: Unconjugated
• Alternative Names: MNK1(phospho T385), p-MNK1(phospho T385), MAP kinase interacting kinase 1, MAP kinase interacting serine/threonine kinase 1, MAP kinase signal integrating kinase 1, MAP kinase signal-integrating kinase 1, MAP kinase-interacting serine/threonine-protein kinase 1, MAPK signal integrating kinase 1, MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1, MKNK1, MKNK1_HUMAN, MNK 1, Mnk1.

Product Description

Phospho-MNK1 (Thr385) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 51 kDa
• UniProt: Q9BUB5
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: MKNK1

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Application Notes: Modification: Phosphorylated

Images

Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-MNK1 (Thr385)) Polyclonal Antibody, Unconjugated (orb185175) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p-MNK1 (Thr385)) Polyclonal Antibody, Unconjugated (orb185175) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.