AGL Antibody (N-term), Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1931626
Artikelname: AGL Antibody (N-term), Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1931626
Hersteller Artikelnummer: orb1931626
Alternativnummer: BYT-ORB1931626-100,BYT-ORB1931626-50
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: GDE
AGL Antibody (N-term)
Klonalität: Polyclonal
Molekulargewicht: 174764 Da
UniProt: P35573
Formulierung: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application Verdünnung: WB - 1:1000, IF - 1:10-50
Expression of myc-GS causes wild type but not the ACBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL ACBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.
Anti-AGL Antibody (M15) at 1:1000 dilution + human skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 175 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.