AGL Antibody (N-term), Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: AGL Antibody (N-term), Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB1931626
• Supplier Catalog Number: orb1931626
• Alternative Catalog Number: BYT-ORB1931626-100,BYT-ORB1931626-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: GDE

Product Description

AGL Antibody (N-term)

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 174764 Da
• UniProt: P35573
• Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application Notes

• Application Dilute: WB - 1:1000, IF - 1:10-50

Images

Expression of myc-GS causes wild type but not the ACBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL ACBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.

Anti-AGL Antibody (M15) at 1:1000 dilution + human skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 175 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.