Goat anti-CD80 Antibody, Unconjugated, Polyclonal

Produktübersicht

• Artikelname: Goat anti-CD80 Antibody, Unconjugated, Polyclonal
• Artikelnummer: BYT-ORB20356
• Hersteller Artikelnummer: orb20356
• Alternativnummer: BYT-ORB20356-100
• Hersteller: Biorbyt
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA, FC, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: anti CD80 antibody, anti CD80 molecule antibody, anti CD28LG antibody, anti CD28LG1 antibody, anti LAB7 antibody, anti B-lymphocyte activation antigen B7 antibody, anti CD80 antigen antibody, anti CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody, anti costimulatory factor CD80 antibody, anti costimulatory molecule variant IgV-CD80 antibody

Produktbeschreibung

Goat polyclonal to CD80

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 33
• Puffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
• Sequenz: HLRVNQTFNWNTTK
• Target-Kategorie: CD80

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:4000, WB: 0.3-1 µg/ml
• Anwendungsbeschreibung: Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 60kDa band observed in Human Lymph Node and Human Tonsil lysates (calculated MW of 33.0kDa according to NP_005182.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 µg/ml

Bilder

0.5 µg/ml staining of K562 (A) and U937 (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

3 ug/ml staining of Human Lymph Node (A), Bone Marrow (B) and 1.5 ug/ml Spleen (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

6 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

5 µg/ml staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.