anti CD80 antibody, anti CD80 molecule antibody, anti CD28LG antibody, anti CD28LG1 antibody, anti LAB7 antibody, anti B-lymphocyte activation antigen B7 antibody, anti CD80 antigen antibody, anti CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody, anti costimulatory factor CD80 antibody, anti costimulatory molecule variant IgV-CD80 antibody
Goat polyclonal to CD80
Clonality:
Polyclonal
Molecular Weight:
33
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Sequence:
HLRVNQTFNWNTTK
Target:
CD80
Application Dilute:
ELISA: 1:4000, WB: 0.3-1 µg/ml
Application Notes:
Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 60kDa band observed in Human Lymph Node and Human Tonsil lysates (calculated MW of 33.0kDa according to NP_005182.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 µg/ml
0.5 µg/ml staining of K562 (A) and U937 (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
3 ug/ml staining of Human Lymph Node (A), Bone Marrow (B) and 1.5 ug/ml Spleen (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
6 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
5 µg/ml staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
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