Synaptophysin Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: Synaptophysin Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB313021
• Hersteller Artikelnummer: orb313021
• Alternativnummer: BYT-ORB313021-100, BYT-ORB313021-200, BYT-ORB313021-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: KLH conjugated synthetic peptide derived from human Synaptophysin (151-250/313aa)
• Konjugation: Unconjugated
• Alternative Synonym: MRX96, MRXSYP, XLID96, A230093K24Rik, Syn, p38, Syp1, SYPH_BOVIN, SYP, Major synaptic vesicle protein p38, SYPH_HUMAN, SYPH_MOUSE, BM89 antigen, SYPH_RAT,

Produktbeschreibung

Synaptophysin Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 34 kDa
• UniProt: P08247
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: SYP

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Bilder

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (orb313021) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (orb313021) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (orb313021) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Cerebrum (mouse) Lysate at 40 ug, Primary: Anti-Synaptophysin (orb313021) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.

Immunohistochemical staining of Mouse brain tissue using Synaptophysin antibody.

Immunohistochemical staining of mouse stomach tissue using Synaptophysin antibody.