Synaptophysin Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Synaptophysin Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB313021
• Supplier Catalog Number: orb313021
• Alternative Catalog Number: BYT-ORB313021-100, BYT-ORB313021-200, BYT-ORB313021-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse
• Immunogen: KLH conjugated synthetic peptide derived from human Synaptophysin (151-250/313aa)
• Conjugation: Unconjugated
• Alternative Names: MRX96, MRXSYP, XLID96, A230093K24Rik, Syn, p38, Syp1, SYPH_BOVIN, SYP, Major synaptic vesicle protein p38, SYPH_HUMAN, SYPH_MOUSE, BM89 antigen, SYPH_RAT,

Product Description

Synaptophysin Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 34 kDa
• UniProt: P08247
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: SYP

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (orb313021) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (orb313021) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (orb313021) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Cerebrum (mouse) Lysate at 40 ug, Primary: Anti-Synaptophysin (orb313021) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.

Immunohistochemical staining of Mouse brain tissue using Synaptophysin antibody.

Immunohistochemical staining of mouse stomach tissue using Synaptophysin antibody.